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Characterization and biological properties of sulfated polysaccharides of Corallina officinalis and Pterocladia capillacea Acta Botanica
Ismail,Mona Mohamed; Amer,Mohamed Saleh.
ABSTRACT Red seaweed possess various sulfated polysaccharides (SPs) that could potentially be exploited as bioactive agents for medical and industrial applications. Crude polysaccharides from the red algae Corallina officinalis (SP1) and Pterocladia capillacea (SP2) were extracted and characterized according to their chemical content and their antioxidant, anti-inflammatory, anticoagulant, antibacterial, antifungal, and antifouling activities. The isolated polysaccharides contained low levels of protein and high levels of carbohydrate and sulfate. The extracted SPs were characterized by Fourier-transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectral data and revealed that SP1 is composed of carrageenan, while SP2 is composed of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticoagulant; Antifouling; Antimicrobial; Antioxidant; Polysaccharides; Red seaweed.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400623
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Chemical composition and bioactivity of methanolic extract obtained from Lepista sordida BJPS
Acharya,Krishnendu; Ghosh,Sandipta; Biswas,Rhituparna.
The present study aimed at the evaluation of chemical composition and bioactive potential of methanolic extract obtained from Lepista sordida in terms of antioxidative and antimicrobial efficacy. The macrofungus is recognized for its high nutritional value and medicinal properties. However, to the best of our knowledge bioactivity of its methanolic extract is yet to be explored. In this investigation, quantitative analysis of mycochemicals revealed the extract contained significant amount of phenolic compounds such as phenols and flavonoids. Ascorbic acid was found in higher amount than β carotene and lycopene which were present in vestigial amounts. A phenolic profile was also determined using high performance liquid chromatography that further confirmed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial; Free radical scavenger; High performance liquid chromatography; Mycotaxon; Mycochemicals.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100600
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Chemical Composition and Biological Activities of The Essential Oil And Anatomical Markers Of Lavandula Dentata L. Cultivated In Brazil BABT
Justus,Barbara; Almeida,Valter Paes de; Gonçalves,Melissa Marques; Assunção,Daniele Priscila da Silva Fardin de; Borsato,Debora Maria; Arana,Andres Fernando Montenegro; Maia,Beatriz Helena Lameiro Noronha Sales; Paula,Josiane de Fátima Padilha de; Budel,Jane Manfron; Farago,Paulo Vitor.
ABSTRACT Lavandula dentata, popularly known as lavender, is commonly used in traditional medicine for the treatment of digestive and inflammatory disorders. The objective of this study was to analyzed the chemical oil composition, antioxidant and antimicrobial activities of the essential oil and anatomical markers of the leaf and stem of L. dentata cultivated in South Brazil. Essential oil showed an antioxidant activity similar to rutin and gallic acid when analyzed by phosphomolybdenum method. However, by the free radical DPPH and ABTS methods, it showed a slight potential antioxidant. Essential oil presented 1,8-cineol (63%) as major component, antimicrobial activity against Gram-positive, Gram-negative bacteria strains and Candida albicans, by broth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Antimicrobial; Antioxidant; Lamiaceae; Quality control; Volatile oil.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100441
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Chemical Constituents and Pharmacology properties of Aristolochia triangularis: a south brazilian highly-consumed botanical with multiple bioactivities Anais da ABC (AABC)
OLIVEIRA,SIMONE Q.; KRATZ,JADEL M.; CHAVES,VITOR C.; GUIMARÃES,TATIANA R.; COSTA,DANIELLE T.M.; DIMITRAKOUDI,SAPFO; VONTZALIDOU,ARGYRO; BORDIGNON,SÉRGIO A.L.; SIMIONATO,CESAR P.; STEINDEL,MÁRIO; REGINATTO,FLÁVIO H.; SIMÕES,CLÁUDIA M.O.; SCHENKEL,ELOIR P..
Abstract: Aristolochia triangularis Cham., is one of the most frequently used medicinal plant in Southern Brazil. Preparations containing the leaves and/or stems are traditionally used as anti-inflammatory, diuretic, as well as antidote against snakebites. This study screened A. triangularis extracts, fractions and isolated compounds for different bioactivities. A weak antiproliferative activity against human lung cancer cell line (A549) was observed only for chloroform fraction obtained from stems (CFstems - CC50: 2.93 µg/mL). Also, a moderate antimicrobial activity against Staphylococcus aureus was detected just for chloroform fraction obtained from leaves (CFleaves -13-16 mm inhibition zone). Additionally, two semi-purified fractions (CFstems-4 and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aristolochia triangularis; Antiproliferative; Antiherpes; Antimicrobial; Antiprotozoal; Aristolochic acids.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500801
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Design and characterization of nanocarriers loaded with Levofloxacin for enhanced antimicrobial activity; physicochemical properties, in vitro release and oral acute toxicity BJPS
Siafaka,Panoraia; Okur,Mehmet Evren; Ayla,Şule; Er,Sevda; Cağlar,Emre Şefik; Okur,Neslihan Üstündağ.
Inorganic and carbon based nanomaterials are widely used against several diseases, such as cancer, autoimmune diseases as well as fungi and bacteria colonization. In this work, Santa Barbara Amorphous mesoporous silica (SBA), Halloysite Nanotubes (HNTs) and Multiwalled Carbon Nanotubes (CNTs) were loaded with fluoroquinolone Levofloxacin (LVF) to be applied as antimicrobial agents. The prepared via adsorption nanocarriers were characterized by Fourier-Transformed Spectroscopy, Scanning Electron Microscopy as well as High Pressure liquid Chromatography. In vitro release studies were carried out using Simulated Body Fluid at 37oC and data analyzed by various kinetic models showing slow dissolution over 12-24 hours. Antimicrobial studies showed improved...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbon nanotubes; Santa barbara amorphous; Halloysite nanotubes; Levofloxacin; Toxicity; Antimicrobial.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100514
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Differential distribution and defence involvement of antimicrobial peptides in mussel ArchiMer
Mitta, Guillaume; Vandenbulcke, Franck; Noel, Thierry; Romestand, Bernard; Beauvillain, Jean Claude; Salzet, Michel; Roch, Philippe.
In previous papers, we characterised 3 types of 4-kDa, cysteine-rich, cationic antimicrobial peptides: MGDs (for Mytilus galloprovincialis defensins), mytilins and myticins, which are abundant in the mussel hemocytes. In the present work, me revealed a differential distribution of the cells expressing the different genes. In addition, using confocal and electron microscopy, we confirmed that defensins and mytilins were partially located in different sub-types of circulating hemocytes although the peptides can be located in the same cell, and even in the same granule. We also demonstrated that mytilins exert their microbicidal effect within the cells through the process of phagosome-mytilin granule fusion leading to the co-location of ingested bacteria and...
Tipo: Text Palavras-chave: Innate immunity; Hemocyte; Mussel; Myticin; Mytilin; Defensin; Antimicrobial.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-413.pdf
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EMJH medium with 5-fluorouracil and nalidixic acid associated with serial dilution technique used to recover Leptospira spp from experimentally contaminated bovine semen BJM
Miraglia,Fabiana; Moraes,Zenaide Maria de; Melville,Priscilla Anne; Dias,Ricardo Augusto; Vasconcellos,Silvio Arruda.
Bovine semen experimentally contaminated with Leptospira santarosai serovar Guaricura was submitted to the modified EMJH medium with 5-fluorouracil (300mg/L) and nalidixic acid (20mg/L), named as "selective medium" and using the serial dilution technique, in order to evaluate the percentage of recovery of the added microorganism. The selective EMJH medium was found with higher percentage of recovery of leptospiras and minor losses of samples due to contamination with opportunistic microorganisms than the non-selective EMJH medium: 151/376 (40.0%) of positive growth; and 38/376 (10.0%) contamination and 58/376 (15%) and 129/376 (34.0%), respectively. These results were statistically significant (p<0. 0001; Fisher). Differences were found when the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospires; Culture media; Antimicrobial; Isolation; Semen.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100033
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Endophytic fungi found in association with Bacopa monnieri as potential producers of industrial enzymes and antimicrobial bioactive compounds BABT
Katoch,Meenu; Salgotra,Aseem; Singh,Gurpreet.
This study aimed to screen the endophytic fungal species of ethano-medicinal plant Bacopa monnieri (L.) Pennell for their ability to produce antimicrobial substances against Bacillus subtilis, Pseudomonas aeroginosa, Salmonella typhimurium, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Candida albicans. Endophytes were also screened for their ability to produce amylase, cellulase, protease and lipase to evaluate their ecological role within the host plant. Twenty-six endophytes were isolated and seventeen were identified. All the isolated endophytes exhibited amylolytic activity. Lipolytic, cellulolytic, proteolytic activity was shown by 98, 28 and 31% isolates, respectively. Similarly, all the endophytes (100%) exhibited significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacopa monnieri; Antimicrobial; ITS 5.8S rDNA; Lipase; Amylase; Endophyte.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500714
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Epidemiological and microbiological aspects of acute bacterial diarrhea in children from Salvador, Bahia, Brazil BJID
Diniz-Santos,Daniel R.; Santana,José S.; Barretto,Junaura R.; Andrade,Maria Goreth M.; Silva,Luciana R..
In the few cases of acute childhood diarrhea that require antimicrobial therapy, the correct choice of the drug depends on detailed previous knowledge of local strains. In order to establish such parameters in our city, we reviewed the results of all 260 positive stool cultures of children between 0 and 15 years of age during two years at a pediatric tertiary care facility in Salvador, Brazil. Bacterial strains had been presumptively identified by culturing in selective media and by biochemical testing, and their antimicrobial susceptibility patterns were automatically detected by the MicroScan Walkaway System. Data about patients' sex and age, monthly distribution of the cases, pathogens isolated and their antimicrobial resistance patterns were recorded....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diarrhea; Antimicrobial; Resistance; Children; Epidemiology.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100013
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Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities Biological Sciences
Santiago, Juliana Andrade; Cardoso, Maria das Graças; Batista, Luís Roberto; Castro, Evaristo Mauro de; Teixeira, Maria Luisa; Pires, Marinês Ferreira.
The aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil from Chenopodium ambrosioides L. and to determine its secretory structures. The essential oil was extracted through the hydrodistillation technique using a modified Clevenger apparatus (2 hours) and chemically characterized by GC/MS and GC-FID. The antioxidant activity was determined by monitoring the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by the oxidation of the β-carotene/linoleic acid system. The evaluation of antibacterial activity was performed by the agar cavity diffusion technique using the microorganisms Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella Cholerasuis. The characterization of trichomes was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Natural products; Antioxidant assays; Antimicrobial; Trichomes.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28303
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Essential oil of Mitracarpus frigidus as a potent source of bioactive compounds Anais da ABC (AABC)
Fabri,Rodrigo L.; Coimbra,Elaine S.; Almeida,Ana C.; Siqueira,Ezequias P.; Alves,Tânia M.A.; Zani,Carlos L.; Scio,Elita.
In our previous work (Fabri et al. 2009), we showed that different extracts of Mitracarpus frigidus had significant antibacterial, antifungal and leishmanicidal activities. In order to increase our knowledge about this species, this work assesses the chemical composition and the in vitro biological activity of its essential oil. Thus, the essential oil obtained by hydrodistillation of the aerial parts of M. frigidus was analyzed by GC/MS. Among several compounds detected, 11 were identified, being linalool and eugenol acetate the major components. The essential oil exhibited a moderate antibacterial effect against Staphyloccocus aureus, Bacillus cereus, Pseudomonas aeruginosa and Enterobacter cloacae (MIC 250 µg/mL). On the other hand, it showed a strong...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mitracarpus frigidus; Essential oil; Antimicrobial; Antileishmanial; Cytotoxicity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400021
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Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia Biol. Res.
Paudel,Babita; Bhattarai,Hari Datta; Chan Kim,Il; Lee,Hyoungseok; Sofronov,Roman; Ivanova,Lena; Poryadina,Lena; Yim,Joung Han.
BACKGROUND: Several plants are reported to be produced various biological active compounds. Lichens from the extreme environments such as high altitude, high UV, drought and cold are believed to be synthesized unique types of secondary metabolites than the other one. Several human pathogenic bacteria and fungi have been muted into drug resistant strains. Various synthetic antioxidant compounds have posed carcinogenic effects. This phenomenon needs further research for new effective drugs of natural origin. This manuscript aimed to screen new source of biological active compounds from plants of subarctic origin. RESULTS: A total of 114 plant species, including 80 species of higher plants, 19 species of lichens and 15 species of mosses, were collected from...
Tipo: Journal article Palavras-chave: Antimicrobial; Antioxidant; Brine shrimp; DPPH; Lichen; Moss.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100010
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Evaluation of Antimicrobial Activity of Glucose Oxidase from Aspergillus niger EBL-A and Penicillium notatum BABT
Zia,Muhammad Anjum; Riaz,Ayesha; Rasul,Samreen; Abbas,Rao Zahid.
This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-200 size exclusion chromatography. The crude enzyme extracts of A. niger and P. notatum showed 2.32 and 5.53 U mg-1 specific activities, respectively, which after desalting was 15.52 and 12.05 U mg-1, and after ion exchange and gel filtration chromatography was 29.09 - 62 and 25.72 - 59.37 U mg-1 for A. niger and P. notatum, respectively. The antimicrobial activity was determined by disc diffusion...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose oxidase; Antimicrobial; Aspergillus niger; Penicillium notatum.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600010
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Evaluation of olive leaf extract as a growth promoter on the performance, blood biochemical parameters, and caecal microflora of broiler chickens R. Bras. Zootec.
Erener,Guray; Ocak,Nuh; Ozturk,Ergin; Cankaya,Soner; Ozkanca,Resit; Altop,Aydın.
ABSTRACT The objective of the study was to examine the effects of an alcoholic liquid olive leaf extract (OLE) obtained from fresh leaves on the growth performance, carcass weight, caecal microflora, and some plasma variables, such as triglycerides, total cholesterol, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) cholesterol in broiler chickens. A total of 375 one-day-old male broilers (Ross 308) were randomly divided into five treatments with five replicate pens (15 birds each) per treatment. The birds were fed either a basal diet with no supplement (control), with 75 (OLE75), 150 (OLE150), 300 (OLE300), and 600 (OLE600) mg kg−1 oleuropein, provided by 0.66, 1.33, 2.65, and 5.32 g kg−1 of the OLE. The dietary supplementation of OLE...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial; Feed additive; Oleuropein; Olive leaves; Plant extract; Poultry.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982020000100600
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Evaluation of the use of probiotic (Bacillus subtilis C-3102) as additive to improve performance in broiler chicken diets R. Bras. Zootec.
Nunes,Jaqueline Oliveira; Bertechini,Antonio Gilberto; Brito,Jerônimo Ávito Gonçalves de; Fassani,Édison José; Mesquita,Fabrício Rivelli; Makiyama,Letícia; Meneghetti,Camila.
The objective of this experiment was to evaluate the use of probiotic based upon Bacillus subtilis C-3102 (BS) in broiler diets containing or not conventional additive growth-promoter (AGP). A total of 1,824 one-day-old chicks of the Cobb-500 strain were used and distributed into 48 plots (1.50 × 2.00 m). A completely randomized design with four treatments and 12 repetitions was adopted. The treatments evaluated were: 1- Negative Control - AGP-free diet (NC); 2 - Positive Control, with AGP halquinol 30 mg/kg diet (PC); 3 - NC + BS (3x10(5) cfu/g diet); and 4 - PC + BS (3x10(5) cfu/g diet). The diets were on corn, soybean meal and meat and bone meal basis, formulated according to the nutrient allowances of the Strain Cobb Guide, following a feed program...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial; Meat poultry production; Microbiota; Nutrition.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001100012
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Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI ArchiMer
Gonzalez, Marcelo; Gueguen, Yannick; Destoumieux Garzon, Delphine; Romestand, Bernard; Fievet, Julie; Pugniere, M; Roquet, F; Escoubas, Jean-michel; Vandenbulcke, F; Levy, O; Saune, Laure; Bulet, P; Bachere, Evelyne.
A cDNA sequence with homologies to members of the LPS-binding protein and bactericidal/permeability-increasing protein (BPI) family was identified in the oyster Crassostrea gigas. The recombinant protein was found to bind LIPS, to display bactericidal activity against Escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. This indicated that it is a BPI rather than an LPS-binding protein. By in situ hybridization, the expression of the C gigas BPI (Cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. Thus, Cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle,...
Tipo: Text Palavras-chave: Oyster innate immunity; Mollusk; Hemocyte; Epithelia; Antimicrobial.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3564.pdf
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Identification and antimicrobial susceptibility patterns of Staphylococcus spp. isolated from canine chronic otitis externa Arq. Bras. Med. Vet. Zootec.
Silva,N..
Swab samples obtained from 96 dogs with chronic otitis externa were cultured for the isolation of Staphylococcus species. Of 57 staphylococcal strains, 41 (72%) were coagulase-negative (CNS). The identification of staphylococci strains was made by standard procedures for the routine identification of staphylococci in clinical practice. S. sciuri was the most frequent species isolated (22.8%) from chronic otitis externa in dogs followed by S. intermedius (12.3%), S. auricularis (10.5%) and S. aureus (8.8%). Three (5.2%) CNS strains could not be identified. Bacterial isolates were susceptible to enrofloxacin, gentamicin, cephalothin, chloramphenicol and neomycin. Resistance was most common to penicillin G, oxacillin and ampicillin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Otitis externa; Staphylococci; Antimicrobial.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000200001
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Improving the Antioxidant Properties of Calophyllum inophyllum Seed Oil from French Polynesia: Development and Biological Applications of Resinous Ethanol-Soluble Extracts ArchiMer
Cassien, Mathieu; Mercier, Anne; Thétiot-laurent, Sophie; Culcasi, Marcel; Ricquebourg, Emilie; Asteian, Alice; Herbette, Gaëtan; Bianchini, Jean-pierre; Raharivelomanana, Phila; Pietri, Sylvia.
Tamanu oil from Calophyllum inophyllum L. has long been used in traditional medicine. Ethanol extraction was found the best strategy for recovering bioactive compounds from the resin part of Tamanu oil, yielding two neutral and acidic resins fractions with high phenolics, flavonoids and pyranocoumarins concentrations. A further cascade of LPLC/HPLC separations of neutral and acidic resin fractions allowed identifying fifteen metabolites, and among them, calanolide D and 12-oxocalanolide A (both in neutral fraction) were first identified from a natural source. All these extracts, subfractions and isolated metabolites demonstrated increased free radical scavenging, antioxidant, anti-inflammatory, antimicrobial and antimycobacterial activity compared to...
Tipo: Text Palavras-chave: Calophyllum inophyllum L; Tamanu oil; Ethanol-soluble resin extracts; Lipophilic antioxidants; Phenolics; Pyranocoumarins; Antimicrobial; Anti-inflammatory; UV protection; EPR spin trapping.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00677/78918/81283.pdf
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Influence of subinhibitory concentrations of antimicrobials on hydrophobicity, adherence and ultra-structure of Fusobacterium nucleatum BJM
Okamoto,Ana C.; Gaetti-Jardim Jr.,Elerson; Arana-Chavez,Victor E.; Avila-Campos,Mario J..
Fusobacterium nucleatum is considered a bridge organism between earlier and later colonizers in dental biofilms and a putative periodontopathogen. In Dentistry, antimicrobial agents are used for treatment and control of infectious diseases associated with dental plaque. Antiseptics have been used in association with antibiotics to reduce infections after oral surgeries. In this study, the influence of subinhibitory concentrations (SC) of chlorhexidine, triclosan, penicillin G and metronidazole, on hydrophobicity, adherence to oral epithelial cells, and ultra-structure of F. nucleatum was examined. All isolates were susceptible to chlorhexidine, triclosan, and metronidazole; however, most of the isolates were susceptible to penicillin G, and all of them...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusobacterium nucleatum; Hydrophobicity; Adherence; Antimicrobial; Ultra-structure.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200017
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Intein-mediated expression of cecropin in Escherichia coli Electron. J. Biotechnol.
Díaz,Mauricio; Venturini,Elena; Marchetti,Stefano; Arenas,Gloria; Marshall,Sergio H.
Different strategies have been used to overcome the difficulties to produce antimicrobial peptides. Here we used Intein Mediated Purification with an Affinity Chitin-binding Tag (IMPACT-System, New England Biolabs) for the expression of the antimicrobial peptide cecropin to reduce its sensitivity to intracellular proteases and use its inducible self-cleaving capability to remove the carrier. Cecropin was cloned into suitable expression vector pTYB11, and expression induced by IPTG in Escherichia coli ER2566. The use of 22ºC induction allowed the expression of cecropin with its intein carrier in soluble form. Cell extracts were purified by chitin affinity chromatography and intein-mediated splicing of the target protein was achieved by thiol addition,...
Tipo: Journal article Palavras-chave: Antimicrobial; Cecropin; Fusion; Intein; Peptide; Soluble.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200003
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